Blotting and Hybridization

Blotting and Hybridization

3MW Blotting Paper, Whatman Membranes, & Labnet Hybridization Ovens

		3MW Blotting Paper 3MW Blotting Paper is used widely in nucleic acid and protein research for blotting, wicking, spacers, and gel drying due to its purity, quality and consistent results. Uniform capillary action provides even wicking and enables gel transfers to be lifted reliably from glass supports. Ideal for Western blots, Southern blots, or Northern blots.
		Whatman 3MM Blotting Paper Whatman 3MM Blotting Paper is the world’s most widely used blotting paper. This acceptance and usage reflects the trust of researchers performing Western, Southern, and Northern transfers, as well as for lifting of sequencing gels. Manufactured and tested specifically for chromatography and blotting techniques, these pure cellulose papers ensure the wicking capability, uniformity, and capillary action needed for clean and even transfers during blotting.
		Whatman Nytran Nylon Membranes Whatman Nytran Nylon Membranescome in two formats, Nytran N and Nytran SuPerCharge (SPC). Nytran N is ideal for applications that require a lower charge, and is designed for Southern blotting and Northern blotting as well as colony lifts and plaque lifts. Nytran SPC nylon membranes have a very high positive charge, but are manufactured with a process that allows a combination of high positive charge with low background. Whether using radioactive or non-radioactive detection techniques, Nytran SPC consistently gives high signal with extremely low background.
		Whatman Protran Nitrocellulose Membranes Whatman Protran Nitrocellulose Membranes are the most frequently specified transfer media in the world for a wide range of applications including Western blotting. Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantee superior signal-to-noise ratios, without the need for stringent washing conditions. Unlike PVDF membranes, Protran nitrocellulose requires no methanol pre-wetting step. This makes it the membrane of choice for proteins that prefer aqueous environments.
		Whatman Optitran Nitrocellulose Membranes Whatman Optitran Nitrocellulose Membranes consist of 100% pure nitrocellulose cast onto both sides of an inert polyester support material. The support does not affect transfer conditions, and the combination of flexibility, strength, and excellent signal-to-noise ratios make Optitran ideal for Western blotting or other applications that involve repeated antibody stripping and reprobing.
		Whatman Westran PVDF Membranes Whatman Westran PVDF Membranes are available in two formats, Westran S used for protein sequencing and the Western Clear Signal used for Western blotting. Westran S PVDF is a 0.2um pore size hydrophobic membrane designed specifically for protein sequencing applications. The small pore size of this membrane eliminates "blow-through" and increases protein retention over a wide range of molecular weights. Western Clear Signal PVDF is a 0.45 µm membrane specifically designed for Western blotting and protein dot-blot applications.
		Labnet Problot Hybridization Ovens The Labnet Problot Hybridization Ovens meet the needs of medium to high throughput laboratories with their 6 and 12 hybe bottle capacity. The rotisserie and incubation system in the ProBlot Hybe Ovens provides the perfect conditions for consistent results with blotting applications. Mechanical convection creates an extremely uniform environment by circulating air through the chamber while heat input is controlled by a microprocessor, and the desired agitation level is obtained with the variable speed and adjustable axis rotisserie. The H0600 and H1200A have grounded interior electrical outlet for powering small equipment.
		ProBlot 12S from Labnet More than just a hybridization oven, the ProBlot 12S from Labnet has a shaking mechanism built-in to the base, expanding its use to other applications in which a shaker is needed. Microprocessor control and mechanical convection provide an extremely precise and uniform temperature environment. This makes the H1200-SA hybe oven system ideal for Northern blots and Southern blots as well as culture growth and other applications requiring tightly controlled incubations.