Applications
Ultra-Rapid and Ultra-Sensitive Detection of Proteins in Chemiluminescent Western Blotting
Summary:
The UVP BioSpectrum® Imaging System for high sensitivity detection of proteins in conjunction with the FemtoMax™ chemiluminescent-HRP substrate provides for extremely fast and sensitive detection of proteins by Western blot. We demonstrate the detection of 6 different commonly used epitope tags using Rockland Immunochemicals’s epitope tag specific antibodies and a new 12-epitope tag control marker. The combination of high quality primary and secondary antibodies coupled with the new FemtoMax™ HRP substrate and the BioSpectrum® Imaging System allows the detection of femtogram amounts of target proteins with excellent sensitivity and specificity.
Multiplex NIR Imaging of Protein Blots with the UVP BioSpectrum® System and BioLite™ MultiSpectral Source
Summary:
NIR imaging with the BioSpectrum Imaging System and BioLite MultiSpectral Light Source is fast, efficient, and straightforward. With the extensive range of excitation and emission filters available, researchers can detect and quantify virtually any fluorescent dye, from visible to NIR. Protein blotting is a routine technique for determining the presence or absence of one or more proteins, and can provide additional information on quantity of protein (Gallagher and Wiley, 2008).
Next Generation Gel Imaging with GelRed™ and GelGreen™ Dyes and GelDoc-It® Imaging System
Summary:
By using state-of-the-art dyes and imaging systems, researchers can achieve repeatable, highly detailed documentation and analysis while reducing exposure to toxic materials.
Gel imaging and nucleic acid binding dyes are widely used in today’s life science laboratories to visualize DNA fragments in agarose gels. Ethidium bromide (EtBr) has been the predominant dye used for nucleic acid gel staining for decades because of its low initial price and generally sufficient sensitivity (1,2). However, the safety hazard and costs associated with decontamination and waste disposal can ultimately make the dye expensive to use. For this reason, alternative, safer gel stains were developed by scientists at Biotium, Inc. GelRed™ and GelGreen™ dyes are a new generation of fluorescent nucleic acid gel stains designed to replace the highly toxic EtBr. Three attributes make GelRed and GelGreen dyes superior to EtBr and other EtBr alternatives: low toxicity, high sensitivity, and exceptional stability.